Automated synthesis of [18F]fluorocholine using a modified GE TracerLab module

ABSTRACT

The automated radiosynthesis of [18F]fluorocholine was carried out using a modified reactor design in the GE TracerLab FX(FDG) module. This PET imaging tracer was synthesized in two steps and involved the generation of nucleophilic [18F]fluoride using Kryptofix 2.2.2 technology. The first step was the synthesis of [18F]fluorobromomethane by the reaction of dibromomethane with [18F]fluoride mediated by Kryptofix 2.2.2. Then the substrate N,N-dimethylaminoethanol was N-alkylated with [18F]fluorobromomethane to give the [18F]fluorocholine as the bromide salt. Purification of the salt using Sep-Pak silica gel gave [18F]fluorocholine in a radiochemical purity ≥99% area. This new reactor design in the TracerLab module produced high radiochemical purity and reproducible yields of [18F]fluorocholine. Furthermore, this automated approach could be implemented for routine PET imaging of oncological disease states in the Nuclear Medicine clinical setting.

Keywords: automated radiosynthesis; positron emission tomography; fluorine radioisotopes; choline; 2-deoxy-2-[18F]fluoro-D-glucose; radiopharmaceuticals.

Citation: Sperandeo A, Ficola U, Quartuccio N, Kitson SL, Mansi L, Cistaro A. Automated synthesis of [18F]fluorocholine using a modified GE TracerLab module. Journal of Diagnostic Imaging in Therapy. 2014; 1(1):49-58.

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DOI: http://dx.doi.org/10.17229/jdit.2014-0921-003

Copyright: © 2014 Mansi L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.


Received: 02 September 2014 | Revised: 19 September 2014 | Accepted: 19 September 2014

Published Online 21 September 2014  http://www.openmedscience.com

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JDIT-2014-0921-003